What are sizes of the fragments from an enzyme digestions (endonuclease) of the Lambda Phage plasmid DNA using Eco RI and Pst I? Make sure to have an uncut and double-cut reaction. Explain the mechanism of action of each enzyme and then list the fragment all sizes and draw the results.
Digestion of lambda DNA with Eco RI generated 6 fragments. The Pst I restriction enzyme produces 29 lambda DNA fragments.
What are restriction enzymes?Restriction enzymes are DNA-cutting enzymes found in bacteria. These are site-specific endonucleases also known as molecular scissors.These recognize and cut DNA only at a particular sequence of nucleotides.DNA from the bacteriophage Lambda (48,502 base pairs in length) is cut with a variety of restriction enzymes and the resulting fragments are separated using gel electrophoresis. Size of fragment of uncut lambda DNA is 48502 b.p.Eco RI recognizes G AATTC
CTTAA G
Lambda phage has 5 recogition sites for Eco RI. It cleaves the DNA at ‘G’ of the above sequence.Relative sites of cleavage in base pairs are 21226, 26104, 31747, 39168, 44972.Sizes of fragments produced when the lambda DNA cut with Eco RI 21226, 7421, 5804, 5643, 4878, 3530.Digestion of lambda DNA with EcoRI generated 6 fragments but 2 of them are so close in size that they are difficult to separate.Pst I recognizes CTGCA G
G ACGTC
The Pst I restriction enzyme produces 29 fragments of lambda DNA. It cleaves the DNA at ‘G’ of the above sequence.The Pst I digest of λ DNA yields the following discrete fragments (in base pairs): 11497, 5077, 4749, 4507, 2838, 2556, 2459, 2443, 2140, 1986, 1700, 1159 1093, 805, 514, 468, 448, 339, 264, 247, 216, 211, 200, 164, 150, 94, 87, 72 and 15.The cohesive ends (12b cos site of bacteriophage) of fragments 11501 bp and 2556 bp may anneal and form an additional band.Learn more about restriction enzymes here:
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Do you think the responses from the Sympathetic Nervous System, or the Parasympathetic Nervous System, have had a greater influence on human evolution
What is human nervous system?
The human nervous system is divided into two parts,1. The Central Neural System(CNS)
2. The Peripheral Neural System(PNS)
The CNS includes the brain and the spinal cord and is the site ofinformation processing and control. The PNS comprises of all the nerves of the body associated with the CNS i.e., brain and spinal cord.The nerve fibres of the PNS are of two types :1. Afferent fibres
2. Efferent fibres
The PNS is divided into two divisions called;a. Somatic neural system
b. Autonomic neural system
The somatic neural system relays impulses from the CNS to skeletal muscles while the autonomic neural system transmits impulses from the CNS to the involuntary organs and smooth muscles of the body. The autonomic neural system is further classified as;(i) Sympathetic neural system
(ii) Parasympathetic neural system
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Animals that lie in cold climates tend to have higher proportions of __________ fatty acid residues in their lipids than do animals that live in warm climates.
Answer: polyunsaturated fatty acid residues
Explanation: Unsaturated fatty acids have a lower melting point so arctic animals have these fatty acids in their membranes.
The motor neuron and all the myofibers it innervates is referred to as a ____________ . every time a motor neuron sends a nerve impulse to a skeletal muscle fiber, the muscle fiber will
The motor neuron and all the myofibers it innervates is referred to as a __motor units__ . every time a motor neuron sends a nerve impulse to a skeletal muscle fiber, the muscle fiber will contract .
What is the motor units?
The motor unit is the association between the motor neuron and a group of fibers from the muscle.
A single muscle is composed of several motor units. The fibers composing each of them are just a little part of the whole muscle mass, meaning this is the part that will contract.
The motor unit is the basic unit from which the CNS controls motion.
How does contraction occur?The central nervous system (CNS) generates an action potential in the presynaptic membrane.
This action potential provokes the neurotransmitter release (acetylcholine, Ach) into the synaptic space.
The neurotransmitter travels through the synaptic space and reaches the postsynaptic membrane, where it binds to its receptors on the skeletal muscle fiber, causing ion channels to open.
Ion interchange between the extracellular space and the cell interior triggers a series of events that end by depolarizing the muscle fiber membrane, and leading to fiber contraction.
The motor neuron and all the myofibers it innervates is referred to as a _motor unit_ . every time a motor neuron sends a nerve impulse to a skeletal muscle fiber, the muscle fiber will contract.
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What can be known about Mendel's five-part hypothesis?
Answer: It is an answer to a scientific question and is based mostly on his research.
Explanation:
The fungus ________ can cause a variety of diseases, depending on the condition of the person.
a) sporothrix schenckii
b) amanita phalloides
c) aspergillus gyromitra
d) esculenta mucor
The fungus Aspergillus can cause a variety of diseases, depending on the condition of the person (Option C).
What is Aspergillus?Aspergillus is a type of fungus that grows in different climatic conditions around the world and that is well known because it can survive both in indoors and outdoors environments.
This fungus (Aspergillus) is well known to cause Aspergillosis, which is a disease characterized by distinct symptoms such as shortness of breath, wheezing, fever, and loss of weight.
In conclusion, the fungus Aspergillus can cause a variety of diseases, depending on the condition of the person (Option C).
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Color (black/gray/red) is a ___________ characteristic
Their are three main characteristic is intensity, saturation, and hue .
Red color is motivated by power, black is achromatic color with hue like white gray and white.black is symbol of darkness. Brightly co-loured object is one that reflects and transmits a large portion of light falling on it.
The great amount of light is reflected on white screen while a black screen would not reflects any light. Co-lour also linked with various personality trait , motivation and productivity levels in life. It symbolize life and death , love and evil and happiness and anger.Red color makes you feel passionate and energized.
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Place the following australopithecines in the chronological order in which they likely lived, from oldest to most recent.
Australopithecus anamensis, Australopithecus afarensis, Australopithecus garhi, Australopithecus sediba .
What is the oldest australopithecine?Au. anamensis, the earliest Australopithecus species, was found in northern Kenya, close to Lake Turkana, in Kanapoi and Allia Bay. After examining solitary teeth, upper and lower jaws, skull pieces, and tibia bones discovered at the discovery locations, the species was initially described in 1995.At least seven species of australopithecines are now generally recognized, including Australopithecus afarensis.What are the 7 stages of human evolution?The following are the stages of human evolution:
Dryopithecus- These are deemed to be the ancestors of both man and apes.Ramapithecus.Homo Erectus.Homo Sapiens Neanderthalensis.Homo Sapiens Sapiens.Learn more about australopithecines
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____________________ evolution states that all life on earth is descended from other life and has transformed from, at times, very different ancestors.
Organic evolution states that all life on the earth is descended from from other life and has transformed from , at times , very different ancestors.
Evolutionary theory is based on ideas of species and related to their gradual decreases.It is given by Darwin which is based on natural selection. Evolution is not theory about the origin of life. Its express about that how species change over time and what many people think there is also little conflict between evolution.
Evolution is the process in which gradual change that takes places over many generation to generations in which species of animals , plants or insects slowly change some of their physical characteristics.
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Archaea differ from bacteria in that archaea Group of answer choices Are prokaryotic. Reproduce by binary fission. Lack peptidoglycan. Lack nuclei. Use organic compounds for food.
Archaea differ from bacteria in that archaea lack peptidoglycan.
What about archaea and bacteria?Although archaea lack internal membranes, they do have a cell wall and swim by using flagella. The difference between bacteria and archaea is that bacteria have an ester-linked cell membrane, while archaea have an ether-linked cell membrane.Organelles and other internal membrane-bound structures are not present in the cells of bacteria and archaea. In contrast to eukaryotes, bacteria and archaea do not have a nucleus that separates their genetic material from the rest of the cell.Similar to Eubacteria, Archaea have a polysaccharide and glycoconjugate-rich cell wall. The strong cell borders that archaea form despite not having peptidoglycan make them resistant to high internal osmotic pressure.
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The average person in the united states generates how many pounds of municipal solid waste each day?
Municipal solid waste (MSW) generation in 2018 totalled 292.4 million short tonnes or 4.9 pounds per person daily in US.
According to the EPA, trash, also known as municipal solid waste, is everything that customers discard after using it. These include corrugated boxes and bottles, food, furniture, grass cuttings, computers, tyres, and refrigerators. However, MSW does not incorporate all wastes that might be disposed of locally, including non-hazardous industrial wastes, municipal wastewater sludge, and construction and demolition (C&D) waste debris. EPA has recently begun to include estimates of C&D generation and management as a separate non-hazardous waste stream.
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Does Pterocaulon Plystachyum affect cell division?
Answer:
polystachyum infusions produced the greatest inhibition of onion root-tip cell division and decreased mitotic index but there was no statistically significant intra or interpopulational variability for the P. polystachyum populations from different sites in regard to their cytotoxic effects.
what will happen in absence of semilunar valves?
Answer:
The absence of semilunar valves could cause a fetal death or induced abortion.
Explanation:
Severe regurgitation in both great arteries leads to volume overload in both ventricles, which causes fetal hydrops and death.
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A family took their newborn to the doctor. They were worried something was wrong because the baby seemed to be in constant pain. After medical tests revealed the baby had large amounts of lactic acid accumulating in his muscle cells, doctors diagnosed the child with a rare disease in which mitochondria are missing from skeletal muscle cells. How might the lack of mitochondria explain the large amounts of lactic acid in the baby's muscle cells
Mitochondria help in the complete metabolism of sugar to produce energy in the cell (ATP). Mitochondria is the powerhouse of the cell.
Glycolysis is the first step in the metabolism of glucose. By the process of glycolysis, glucose is converted to pyruvate with the generation of ATP and NADH. The pyruvate is then converted to acetyl CoA by the pyruvate dehydrogenase complex which then enters the TCA cycle. The NADH FADH2 generated by Glycolysis and TCA cycle donate their electrons to the electron transport chain which in turn helps in the generation of ATP. Both the TCA cycle and the Electron transport chain take place within the mitochondria. In the absence of mitochondria, NAD+ and FAD are regenerated by fermentation.
In the process of converting 3-carbon pyruvate to 3-carbon lactic acid (C3H6O3), lactic acid fermentation also regenerates NAD+, which enables glycolysis to continue producing ATP in the absence of mitochondria. This leads to the accumulation of lactic acid in muscle cells.
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The most common reportable disease in the united states is:_______
a. cystitis.
b. lymphogranuloma venereum.
c. gonorrhea.
d. syphilis.
e. candidiasis.
Gonorrhea is a sex-transmitted disease caused by a bacteria that equally infects men and women. Option C.The most common reportable disease in the United States is gonorrhea.
What is gonorrhea?Gonorrhea is transmitted by sexual contact. The disease equally affects men and women, who may or may not experience symptoms. This is a very common affection that affects mostly people between 15 and 25 years old.
More than a million cases of Gonorrhea per year are reported in the United States. Because of the high incidence of the disease, especially in young people, health workers must inform the State Board of Health about all Gonorrhea cases.
The correct option is C. The most common reportable disease in the united states is:_gonorrhea_
Due to technical problems, you will find a more detailed explanation in the attached files.
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!! What is labeled "A" on the chart?
Answer:
can you put the chart
Explanation:
not enough information
What type of covalent bond is responsible for holding together the secondary structure of proteins
Answer :Hydrogen Bonds
Explanation:
Two organisms of different species mate and produce offspring that cannot mate with its siblings or with members of either parental species. This is an example of __________.
Two organisms of different species mate and produce offspring that cannot mate with its siblings or with members of either parental species. This is an example of hybrid infertility.
Hybridization is the technique used to combine two distinct hereditary, which might cooperate in a dissonant way. This sort would be supposed frequently prompt to awful outcomes. The combination in a single organic entity of two genomes that are independently useful will mostly bring about adverse interaction on the grounds that every one of the genes in a single genome should be viable with every one of the genes in the other.
Hybrid infertility and sterility, on the whole known as half-breed incongruence, can diminish the exchange of hereditary variations between species. In this way, hybrid infertility can be significant during the process of speciation by acting as a reproductive inhibitor.
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The correct sequence for the phases of the human cell cycle, starting with a newly divided cell, is?
Starting with a newly divided cell, the correct sequence for the human cell cycle phases are interphase followed by mitosis and then cytokinesis.
Interphase of the cell cycle does not involve any cell division. It is a period of growth and replication of the nuclear DNA. The cell prepares for the division in this stage. Interphase is further divided into the G1, S and G2 phases. Mitosis is the next phase of the cell cycle which involves the division of the cell in order to form daughter cells. Mitosis is further divided into the prophase, metaphase, anaphase and the telophase. Cytokinesis marks the end of the mitosis and this is where the cell actually separates to produce two daughter cells.
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You are part of a research group experimenting with signaling molecules that would tell cancer cells to stop dividing. The cancer cells have inactive receptors on their plasma membrane so you need an alternate method of getting the signals into the cells. What could you use
Gap junctions for direct contact is used to cancer cells have inactive receptors on their plasma membrane so you need an alternate method of getting the signals into the cells.
What about cancer cell?The MAP kinase route, which controls cell proliferation, and the phosphoinositide 3-kinase (PI3K) pathwayIt controls cell metabolism and survival, are the two RAS signaling pathways that are most frequently linked to cancer.Signaling pathways are affected by cancer-causing mutations. The genomic changes in cancer cells can be linked to signaling pathways that regulate tumor-related functions and put in the perspective of distorted larger signaling networks that promote cancer progression.To transmit signals from the extracellular environment to the cell nucleus, where they activate particular genes necessary for cell division, growth, and differentiation.Learn more about gap junction here:
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In a hardy-weinberg population with two alleles, a and a, that are in equilibrium, the frequency of the allele a is 0. 3. What is the frequency of individuals that are homozygous for this allele?.
Here, given-
homozygous alleles 'a' have a frequency of 0.3.
Also the alleles are in equilibrium in a Hardy-Weinberg population. The frequency of individuals that are homozygous for this allele are= 0.49.
The Hardy-Weinberg equilibrium can be defined as the principle which states that the variation in the genetic makeup of a population remains constant and unchanged till there are no external interferences, influencing the population.
Calculation-
[tex]p + q= 1\\0.3 + q= 1\\q= 1- 0.3\\q= 0.7\\[/tex]
Then to find the frequency of the individuals homozygous for this allele the following formula needs to be used-
[tex]p^{2} + 2pq + q^{2} = 1\\Here,\\p^{2}= dominant homozygous frequency\\2pq= heterozygous frequency\\q^{2}= recessive homozygous frequency\\[/tex]
Thus, the individuals homozygous for the allele can be calculated by-
[tex]q^{2} = (0.7)^{2} \\q^{2}= 0.49[/tex]
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When any of your body cells becomes infected with a pathogen, to what protein do the foreign peptides become attached that initiates the immune response
Interferons
Virally infected cells produce and release small proteins called interferons, which play a role in immune protection against viruses.
What are Interferons ?Interferons prevent replication of viruses, by directly interfering with their ability to replicate within an infected cell.
They also act as signalling molecules that allow infected cells to warn nearby cells of a viral presence – this signal makes neighbouring cells increase the numbers of MHC class I molecules upon their surfacesSo that T cells surveying the area can identify and eliminate the viral infection.Learn more about Interferons here:
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Misha often experiences severe swelling and joint pain in her fingers and wrists each morning. this pattern of symptoms is most likely due to:______
Misha often experiences severe swelling and joint pain in her fingers and wrists each morning. This pattern of symptoms is most likely due to Rheumatoid arthritis (RA).
Rheumatoid arthritis is an autoimmune disorder and inflammatory condition in which your immune system unintentionally assaults healthy cells in your body, leading to painful swelling(inflammation) in the areas of your body affected. It primarily targets joints, typically several joints at once. Hand, fingers, wrist, and knee joints are frequently impacted. Joint tissue is harmed in a Rheumatoid arthritis-affected joint because of the inflammation of the joint lining resulting in Long-lasting or persistent pain.
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What types of cells are the primary targets of the healthy immune system? select all that apply.
Infectious, foreign, or cancerous cells are the primary targets of a healthy immune system.
White blood cells also known as leukocytes, play a vital role in the immune system. Immune cells are developed from stem cells in the bone marrow and can be divided into different types. Neutrophils, eosinophils, basophils, mast cells, monocytes, macrophages, dendritic cells, natural killer cells, and lymphocytes all are types of white blood cells.
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Unlike other saturated fatty acids, the specific types of saturated fatty acids in coconut oil may _______ blood levels of hdl cholesterol.
Unlike other saturated fatty acids, the specific types of saturated fatty acids in coconut oil may increase blood levels of HDL cholesterol.
What is saturated fatty acids?Fatty acids are found in many plants and animals and they abound as part of out diet such as in butter fat, meat fat etc. These are the fatty acids that are composed only of a long chain of carbon atoms that do not contain multiple bonds.
We can classify fatty acids as saturated or unsaturated based on the presence or the absence of the multiple carbon bonds on the molecular structure of the compound.
Hence, unlike other saturated fatty acids, the specific types of saturated fatty acids in coconut oil may increase blood levels of HDL cholesterol.
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Which of the following statements is correct with respect to the use of oxidized sulfate by some bacterial groups
All of the above.
What are sulfur oxidizing bacteria?Any of a wide range of microorganisms that can metabolize sulfur and its components and are significant in nature's sulfur cycle are known as sulfur bacteria. Hydrogen sulfide (H2S), sulfur, and thiosulfate are a few of the typical sulfur compounds that are used by these bacteria as a source of energy. Sulfate is the end result of the oxidation of sulfur.
In deep earth deposits, Thiobacillus, which is common in both marine and terrestrial settings, produces sulfuric acid, which dissolves metals in mines but also corrodes concrete and steel. It also oxidizes sulfur, generating sulfates that are helpful to plants.
I understand the question you are looking for is this:
In seawater, sulfate is the most common anion after chloride. Which of the following is correct with respect to the use of sulfate by some bacterial groups?
Oxidized sulfur-containing molecules have redox potentials lower than those of the nitrogen series.Oxidized sulfur molecules such as sulfate and sulfite serve as electron acceptors.Sulfate and sulfite can receive electrons from hydrocarbons.Sulfate-reducing archaea and bacteria are widespread in the ocean.All of the above.Learn more about sulfur bacteria here:
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What type of trait in mendel's studies could vanish in one generation and reappear in the next generation?
Recessive traits often seem to disappear because two recessive alleles are needed to produce the recessive phenotype. They can skip a generation and then reappear if an individual inherits two copies of the recessive gene. Mendel's experiments revealed that phenotypes could be hidden in one generation, only to reemerge in subsequent generations.
Recessive traits disappear because recessive alleles can hide out in heterozygotes, allowing them to persist in gene pools and natural selection can only see the phenotype, not the genotype. While harmful recessive alleles will be selected against and it's almost impossible for recessive alleles to completely disappear from a gene pool.
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What is the most likely effect of a mutation that changes the repeat sequence found in terc from 3’-cccaau-5’ to 3’-ucaggacg-5’?.
If the repeat sequence of telomere changes from 3’-cccaau-5’ to 3’-ucaggacg-5’ due to any mutation then the telomerase enzyme will be unable to attach to the 3' overhung of the telomere sequence and cannot elongate new DNA strand [telomeric part] as a result, the telomeres of chromosome cannot be elongated and the entire chromosome or the cell will be lost after few rounds of division.
Mutation: This can be described as a random change in the nucleotide sequence due to which the protein sequences change hindering the function performed by the particular protein.
Telomere: They are short repeating sequences found at the end of the chromosome whose function is to save the chromosomes from degradation.
But after each round of replication, the length of the telomeres becomes shorter than the previous and after a certain number of cell divisions, the telomeres become so short that they trigger the cell to stop dividing or to self-destruct (undergo apoptosis).
So, to prevent the shortening of telomeres, the TERC gene codes a telomerase enzyme which is a type of reverse transcriptase. After every cell division, the telomerase enzyme gets attached to the 3' end of the shortened telomere end and synthesizes the complementary DNA to the RNA part of the enzyme until the length of the telomere is restored again.
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what are biomolecules
(*´ω`*)
Explanation:
Definition: A biomolecule is a chemical compound found in living organisms. These include chemicals that are composed of mainly carbon, hydrogen, oxygen, nitrogen, sulfur and phosphorus. Biomolecules are the building blocks of life and perform important functions in living organisms
The bacteria, Angelinus ballerinea secretes a compound that inhibits the growth of other Gram-positive bacteria. Scientists take this compound and add chemical groups to it to make it more stable for use in humans to treat bacterial infections. This is an example of a(n)
The bacteria, Angelinus ballerinea secretes a compound that inhibits the growth of other Gram-positive bacteria. Scientists take this compound and add chemical groups to it to make it more stable for use in humans to treat bacterial infections. This is an example of antibiotic.
What is antibiotic?Antibiotics from the key class of glycopeptides can stop this process. Through five H-bonds, these antibiotics bind to the C-terminal d-Ala-d-Ala of the murein precursor, lipid II, and immature peptidoglycan, preventing transglycosylation and/or transpeptidation during the production of the cell wall.Contrarily, antibiotics have easier access to the thick, porous peptidoglycan layer in the cell walls of Gram-positive bacteria, allowing them to more easily enter the cell and/or interact with the peptidoglycan itself.The two main antibiotics that prevent the synthesis of bacterial cell walls are penicillins and cephalosporins.Penicillin is one of many antibiotics that assault the bacterial cell wall in order to operate. The medications specifically stop the bacteria from producing peptidoglycan, a chemical that gives the cell wall the toughness it needs to live in the human body.
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