Staphylococcus bacteria are commonly present in the human nasal cavity and rarely cause disease in the upper respiratory system. Which type of symbiosis does this represent

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Answer 1

Staphylococcus bacteria are commonly present in the human nasal cavity and rarely cause disease in the upper respiratory system. Which type of symbiosis does this represent commensalism.

What is staphylococcus bacteria?Numerous virulence factors, including hemolysins, leukocidins, proteases, enterotoxins, exfoliative toxins, and immune-modulating substances, are produced by the bacterium aureus. During growth, these factors' expression is strictly controlled.Molecules with phagocyte-destructive potential. The measles virus has the ability to weaken the immune system, deactivating host defenses.Numerous poisons, immune evasion mechanisms, and a wide range of protein.Non-protein substances that promote host colonization during infection are among them.

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Related Questions

The cell you are examining under the microscope appears to contain a nucleus. This organism belongs to the domain:________

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The cell you are examining under the microscope appears to contain a nucleus. This organism belongs to the domain Eukarya.

The only domain that includes multicellular, visible organisms including humans, animals, plants, and trees is eukaryote. Numerous microorganisms, including fungi, algae, and micro-animals, also call it home. Since fungi are so diverse, two distinct fungi may have genetic differences comparable to those between a person and a fish.Eukarya are eukaryotes, which have organelles and nuclei that are bound to membranes. Prokaryotes are older, less numerous, and simpler than eukaryotes.Eukarya refers to creatures with nuclei in their cells. Additionally, it is the only realm in which multicellular observable organisms, such as people, animals, plants, and trees, exist. Achaea and bacteria are unicellular organisms without nuclei.

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Which is required for both anaerobic respiration and aerobic respiration?
© oxygen
• water
• mitochondria
• glucose

Answers

the answer is oxygen
Oxygen is the correct answer

During a mumps outbreak at a local school, a teacher has been exposed. the client has previously been immunized for mumps, and consequently possesses:_______

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During a mumps outbreak at a local school, a teacher has been exposed. the client has previously been immunized for mumps, and consequently possesses acquired immunity.

What does acquired immunity means?

Acquired (adaptive or specific) immunity is not present at birth. It is learned.  When a person's immune system comes into contact with foreign invaders and detects nonself substances, the learning process begins (antigens). The components of acquired immunity then discover the most effective strategy for combating each antigen and start to form a memory for that antigen. Specific immunity is another name for acquired immunity. Lymphocytes are the white blood cells that provide innate immunity (T cells and B cells).

After being exposed to a novel antigen for the first time, acquired immunity takes time to develop. However, subsequent reactions to the antigen are quicker and more efficient than those that followed the initial exposure because the antigen is recalled.

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How is an industrial society likely to address the problem of producing too
much air pollution?
A. By cutting down forests to produce more pastureland
B. By developing and using wind energy technologies
C. By requiring licenses for hunting and fishing
D. By having power plants switch from burning coal to burning oil

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Answer:

B. By developing and using wind energy technologies

Explanation:

Wind energy is a clean and renewable resource that can help to reduce air pollution. Wind turbines work by converting the kinetic energy of the wind into electrical energy, which can then be used to power homes and businesses. By increasing the use of wind energy, we can reduce our reliance on polluting fossil fuels and help to create a cleaner, healthier world.

The punnett square below represents a cross between two parents that are heterozygous for the f gene. What percentage of the offspring are predicted to be heterozygous for the f gene?

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50% percentage of the offspring are predicted to be heterozygous for the f gene.

Heterozygous for F means one of the genes is F and the other f. As we can see from the square there is a 50% chance of the offspring has the heterozygous F gene.

If the two versions are one-of-a-kind, you've got a heterozygous genotype for that gene. For an instance, being heterozygous for hair color may want to suggest you have one allele for pink hair and one allele for brown hair. The connection among the 2 alleles impacts which tendencies are expressed.

Heterozygous is a nation of getting inherited one of a kind kinds of a specific gene from each one all your biological parents. Now, through distinct forms, we typically mean that there are different quantities of the gene in which the collection is distinct.

All of us have two alleles, or versions, of every gene. Being homozygous for a specific gene manner you inherited the same variations. It is the opposite of a heterozygous genotype, wherein the alleles are exclusive. Humans who've recessive trends, like blue eyes or pink hair, are continually homozygous for that gene.

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Looking like leafless branches of a tree, the _____ are the primary receivers of signals from other neurons, although the _____ also receives signals directly.

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Looking like leafless branches of a tree, the dendrites are the primary receivers of signals, although the soma also receives signals directly.

Primary receivers of signals from other neurons:

In order to enhance the surface area of the cell body, dendrites, which resemble tree-like extensions, are found at the beginning of neurons. These little protrusions relay electrical stimulation to the soma and take in information from neighboring neurons. Synapses are also found on dendrites.

Along its axon, the signal that is sent to other neurons travels. Even though a neuron may contain millions of dendrites, it will only have one axon. Axon terminals, the fourth separate component of a neuron, are located at the tip of the axon.

Synaptic connections between neurons allow for the transmission of signals in the form of chemical messengers. Action potentials, a type of electrical event, and chemical neurotransmitters are two ways that neurons can communicate with one another.

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A tonicity with a high concentration of solutes is said to be _________. isotonic selectively permeable hypertonic hypotonic

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A tonicity with a high concentration of solutes is said to be Hypertonic.

Option (c) is correct.

The effective osmotic pressure gradient, or the difference in water potential between two fluids separated by a semi-permeable cell membrane, is measured by tonicity.

The relative concentration of specific, membrane-impermeable solutes across a cell membrane, which controls the direction and magnitude of osmotic flux, is what determines tonicity.

In comparison to other solutions, a hypertonic solution contains a higher concentration of non-permeating solutes.

A hypotonic solution contains fewer solutes than another solution.

When the effective osmole concentration of one solution and another solution match, the two solutions are said to be isotonic.

Hence, A tonicity with a high concentration of solutes is said to be hypertonic.

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We used the enzyme, taq polymerase, which is derived from the thermus aquaticus bacterium, in our pcr procedure. Why is this enzyme important for efficiency in pcr?.

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Taq polymerase enzyme important for efficiency in PCR because it is thermostable polymerases that remain active at high temperatures (90 -95  degree Celsius) a beyond the denaturation temperature of DNA.

PCR :

Polymerase chain reaction (PCR) is a laboratory technique for rapidly amplifying millions to billions of copies of a specific segment of DNA, which can then be used for further research.

Taq polymerase is a DNA polymerase derived from bacteria called Thermus aquaticus, which are naturally thermophilic and is a thermostable polymerases that remain active at temperatures higher than the temperature at which DNA is denatured. This allows PCR to be used on DNA samples without the need to add new DNA polymerases after each cycle.

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The most critical element of the biofeedback process is: Group of answer choices change in rate of breathing. immediate information on the status of a biological system. an understanding of how and why the process occurs. change in heart rate.

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b. immediate information on the status of a biological system.

What does a biological system mean?

A biological system is a collection of organs that cooperate to carry out a certain task. For instance, the circulatory system transports nutrients and waste products to and from the body's cells, while the skeletal system protects and supports the body.

Cells, organelles, macromolecular complexes, and regulatory pathways are examples of biological systems that exist on the micro to nanoscopic scale. A living system, such as an organism, should not be confused with a biological system.

The aim of systems biology is to understand vastly complex biological systems in a way that enables us to model and predict how they will behave.

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What is the most likely basis for future human evolutionary change?

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The most likely basis for future human evolutionary change is technological change.

Evolution is a natural process that takes millions of years to occur. In this, an organism evolves on the basis of changing environment, living conditions, availability of food, living place and pattern, etc.

Natural selection works as an important force in this process as nature selects the fittest individuals which means nature selects those individuals who have the most appropriate genes according to the current conditions for survival to reproduce and pass those genes to the next generation.

The differences in the genes depend on an individual's ability to adapt to the changes in the environment and change their living style and habits accordingly to survive.

Therefore, in the current scenario, technology is heading to be the world leader and humans have to learn to adapt to the changes accordingly and evolve in the same way so that they better fit with the coming changes and can survive easily.

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This glaucoma treatment may be used to create a new opening for the escape of aqueous humor from the anterior cavity of the eye, thus relieving intraocular pressure:

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This glaucoma treatment may be used to create a new opening for the escape of aqueous humor from the anterior cavity of the eye, thus relieving intraocular pressure: trabeculectomy

What does Glaucoma mean?

A collection of eye conditions known as glaucoma can result in vision loss and blindness by harming the optic nerve, a nerve located in the back of the eye. You may not notice the symptoms at first because they can appear gradually. Getting a thorough dilated eye exam is the only method to determine if you have glaucoma.

Glaucoma is a lifelong condition and should be referred to as a permanent disorder because it can be managed conservatively.

The procedure of treating glaucoma with surgery is known as trabeculectomy. Trabeculectomy is a filtering surgery where an ostium is created into the anterior chamber from underneath a partial thickness scleral flap to allow for aqueous flow out of the eye.

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please i need help right now!

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Oxygen buddy. Ahahahahaha

Innovations that target NEW markets and represent a major break from existing technologies or ways of thinking, such as the digital camera, the touch-screen smart phone, or the decoding of the human genome, are examples of ______ innovations.

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Innovations that target NEW markets and represent a major break from existing technologies or ways of thinking, such as the digital camera, the touch-screen smartphone, or the decoding of the human genome, are examples of Radical innovations.

What is radical innovation?

An invention that completely alters or replaces an existing business paradigm is considered radical innovation. Radical innovation, as opposed to architectural or incremental innovation, destroys the current system or process and replaces it with something wholly new.

Examples of radical innovation include the iPhone, which helped to create the present smartphone market, and the integration of sensor technology into farming equipment to provide farmers data they may use to change the farming sector.

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Congestive heart failure results from the heart's inability to pump blood. as a result, _____.

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Congestive heart failure results from the heart's inability to pump blood and as a result, fluid accumulates in the body.

What is congestive heart failure?

Congestive heart failure is a condition where the heat cannot pump blood during a prolonged period of time, which is associated with severe health problems.

Congestive heart failure may lead to serious conditions and therefore it must be treated immediately in appropriate clinical settings.

In conclusion, congestive heart failure results from the heart's inability to pump blood and as a result, fluid accumulates in the body (it is known as edema).

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In your Biological Anthropology class, you are given photographs of a fossil. It has a postorbital bar and convergent eyes as well as a relatively large brain. What might you conclude about this fossil

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The fossil belongs to primates.

Primates are an order that belongs to the class Mammalia, which includes animals like monkeys, lemurs, apes, humans, etc.

Primates are a diverse group of animals that cannot be distinguished by just one trait instead they can be distinguished based on an array of characters.

Some distinguishing characteristics primates possess are:

A bony ring structure to protect the side of the eyes from the muscles involved in chewing is known as the postorbital bar.All primates have convergent eyes which means that both the eyes have overlapping visual fields and only allow vision in front.Most primates have trichromatic vision.Primates possess relatively larger brains compared to other mammals. They also have a more complex brain.

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Mycoplasmas differ from other bacteria in that they Group of answer choices Lack a cell wall. Are acid-fast. Are motile. Are gram-negative. Grow inside host cells.

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Mycoplasmas differ from other bacteria in that they Lack a cell wall.

Mycoplasmas are different from bacteria as it can cause a wide range of infections and diseases. Mycoplasmas are phenotypically distinguished from other bacteria by their small size and complete absence of a cell wall. Lack of cell wall can be used to separate the mycoplasms from bacteria. It is a genus of bacteria that, like other members of the class Mollicutes, lack a cell wall around their cell membranes. Peptidoglycan is absent. This property makes them naturally resistant to antibiotics that target cell wall synthesis. They can be parasitic or saprotrophic

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According to the results for Patient 4 shown above, which antigens were present in Patient 4’s blood? Check all (if any) that apply. A antigen B antigen Rh antigen

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A its because you want me and i was just going on with my mom is that you want me and i so the fore its A

Any substance that triggers the production of antibodies by your immune system is referred to as an antigen.

What is antigen?

This indicates that your immune system is attempting to combat the chemical because it does not recognize it.

A substance from the environment, such as chemicals, germs, viruses, or pollen, can behave as an antigen. Inside the body, an antigen may also develop.

Antigens are typically proteins or sugars (polysaccharides) that are present on the surface of various entities, such as viruses or cells. Your immune system recognizes each one by its distinctive shape to determine if it belongs in your body.

Therefore, Any substance that triggers the production of antibodies by your immune system is referred to as an antigen.

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What is the difference between the taxonomic family (biblical kind) and species?

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The differences between the taxonomic family (biblical kind) and species are:-

A species is simply a species or species of organism. For example, all humans belong to one species (our species has the scientific name Homo sapiens) and are different from other species such as gorillas, dogs, and dandelions. However, species definition, identification, and distinction are not so easy.

Kindness is not selfish, compassionate and benevolent. His greatest power was actually revealed to our enemies, and among the fewest. Love your next Show kindness to everything. For the perfect symbol of Bible goodness, we do not need to look beyond Jesus

Species are often defined as a group of organisms that can breed naturally with each other and produce fertile offspring.

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Along the production possibilities frontier, the most efficient point of production depicted is:_______

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Along the production possibilities frontier the efficient point of production frontier , the most efficient point of production depicted is all points on the production possibilities frontier are equally efficient.

Point that strictly lie on the left side of the curve that to be efficient because existing resources would allow for production of more of at least one good without sacrificing the production. The production of most efficient point which curve is the midpoint on the curve .

The base of the average total cost curve in which marginal cost equals average total cost. A formula that is used to measure the percentage of planned production, in order to reduce cost and optimize processes.

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Why is it that intermediate filaments have identical ends and lack polarity, whereas actin filaments and microtubules have two distinct ends with a defined polarity

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The three different types of filaments differ in terms of polarity due to their constituent parts, called soluble subunits.

What is intermediate filaments?The intermediate filament's subunits are elongated, not spherical, and they are linked together in an antipolar way. Because of this lack of polarity in the overall filament, no motor proteins can move along intermediate filaments. Only complex multicellular creatures contain intermediate filaments.Intermediate filaments are more durable and essentially serve as the cell's structural framework.Because the tubulin subunits that make up the microtubule are heterodimers of alpha and beta tubulin, the microtubule is structurally polar. The microtubule's lattice is completely polarized along its whole length, not just at its two ends.

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For each offspring genotype in the Punnett square you just completed, determine the phenotype. In other words, what is the predicted fur color and eye color of the offspring? Using your Punnett square from the last step, fill in the predicted fraction for each phenotype in the data table below.
Black Fur and Black Eyes Black Fur and Red Eyes White Fur and Black Eyes White Fur and Red Eyes

Answers

This was the answer for me on Edge.

Based on the Punnett square, all offspring are predicted to have black fur, with a 50% chance of having black eyes and a 50% chance of having red eyes.

What is a phenotype?

Assuming that black fur (B) is dominant over white fur (b) and black eyes (E) are dominant over red eyes (e), the predicted phenotypes for each genotype in the Punnett square are:

BBEE: Black fur and black eyes

BBEe: Black fur and red eyes

BbEE: Black fur and black eyes

BbEe: Black fur and red eyes

bbEE: White fur and black eyes

bbEe: White fur and red eyes

Using these predicted phenotypes, the data table would be filled in as follows:

Phenotype                     Predicted Fraction

Black Fur and Black Eyes 0.50

Black Fur and Red Eyes 0.50

White Fur and Black Eyes 0.00

White Fur and Red Eyes 0.00

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Which section of the pre-mrna contains the protein-coding regions

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The exons of the pre -mRNA contain the protein -coding regions.

Pre mRNA is the first made mRNA transcript and requires undergo many post transcriptional modifications for the formation of a mature mRNA.

The exons are the regions of the pre-mRNA that are found in the mature RNA, after the splicing of introns takes place.

RNA splicing is the process in which the non-coding segments of the RNA, which are known as introns, are removed by the help of small nuclear ribonucleoproteins.  These SnRNPs make the spliceosome, which catalyzes the process of splicing.

After the introns are removed by the splicing process, the exons are covalently joined, which forms the mature mRNA.

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During the soybean experiment the nongerminating soybeans consumed around 0.02 mL/min of oxygen. What does this tell you about the cellular respiration of the nongerminating soybeans

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Cellular respiration was occurring, but not in sufficient quantities to support growth.

What is germinating soybean?Soybeans that are germinating will have a higher rate of cellular respiration than soybeans that are not germinating.Glycolysis is the first step in cellular respiration and can take place with or without oxygen. Anaerobic respiration is cellular respiration that takes place without oxygen. Aerobic respiration is the type of cellular respiration that takes place when there is oxygen present.Because they are growing and are more active than non-germinating peas, peas that are in the process of germination utilize more oxygen.

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Type your response in the box.
Some of the most promising treatments for cancer are targeted therapies. Use information from
credible websites to explain how targeted cancer therapies differ from traditional chemotherapies,
and describe why these therapies are an advancement in medicinal chemistry.

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Targeted therapies differ from traditional chemotherapy because they are based on specifc cells to treat cancer.

What are Targeted therapies?

Targeted therapies are personalized therapies used in precision medicine whose objective is to treat a health concern by killing specific injured cells.

Conversely, chemotherapy is not specific for cancer cells and this treatment can also kill normal cells.

In conclusion, targeted therapies differ from chemotherapy because they are based on specifc cells.

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How The Earth Was Made: Video viewing guide1) According to Dr. Durda, where did the water that now covers our oceans come from?2) what were the two major gases in Earth's atmosphere 4 billion years ago?

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Billions years ago ocean is formed , water is remained a gas until the earth cooled 212 degree Fahrenheit below.

According to Dr. Durda that water that comes from earth cycles from the oceans ,in earth water cycle  is evaporates from the surface to the atmosphere then form clouds, then fall onto the land as a rain, after rain fall it travels to rivers, lakes and underground .

The two major gases in earth's atmosphere 4 billion year ago the most abundant naturally occcuring gas is oxygen 21% which is second most abundant gas in mature and nitrogen 78% .

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A white blood cell (WBC) count is performed on a blood specimen from a newborn using a hematology analyzer that does not have the capability to detect and quantify nucleated red blood cells (NRBCs). The WBC count that is reported by the analyzer is 28.2 x 109/L, a

Answers

The action that should be taken is that:

The person needs to count the number of cells that looks like the cells in the image attached (that is per 100 WBCs) and then they need to put together the corrected WBC count through the use of this formula.

The Total WBCs x 100 / 100+ the number of NRBCs.

What does white blood cell count tell a person?

A white blood count is known to be a factor that is often used to measures the rate or the number of white cells that is present in your blood.

Note that the White blood cells are a key aspect of the immune system and as such, the action that should be taken is that:

The person needs to count the number of cells that looks like the cells in the image attached (that is per 100 WBCs) and then they need to put together the corrected WBC count through the use of this formula.

See full question below

A white blood cell (WBC) count is performed on a blood specimen from a newborn using a hematology analyzer that does not have the capability to detect and quantify nucleated red blood cells (NRBCs). The WBC count that is reported by the analyzer is 28.2 x 109/L and it is flagged for a manual differential. Upon review of the blood film, more than 6 nucleated cells that resemble the cells shown in the image were identified in the 100 WBC differential. What action should be taken?

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Now that you have had a chance to delve into epigenetics, think about how it applies to the argument between those who say we are a product of our nature and those who think we are nurtured into what we become. Your essay should be at least 250 words and include three different ways epigenetics affects our genes. Write your essay in a separate text document and upload it for submission here

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What we eat (our environment) and genetic variables come together in epigenetics.

Describe epigenetics.

A branch of genetics called epigenetics investigates how variations in the environment might affect how genes are expressed.

DNA methylation, histone alterations, and non-coding RNA pathways are examples of epigenetic mechanisms.

In conclusion, epigenetics is the fusion of genetics and environment.

For most people, perception and the creation of images are usually handled by the:____.
a. left hemisphere of the cortex
b. entire cortex
c. midbrain
d. right hemisphere of the cortex

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The perception and the creation of images are usually handled by the right hemisphere of the cortex . The correct answer is  d. right hemisphere of the cortex.

The brain is the central nervous system's most important organ. It is a complicated organ made up of a vast number of linked glia and neurons. The brain is divided into distinct lobes into each of its two sides. Each lobe performs a distinct set of tasks. The right brain is more intuitive and visually oriented. It is occasionally referred to as the analogue brain. It thinks less logically and with more creativity.

According to a study by Sperry, the right brain assists people in:

imagination holistic thinking arts, rhythm, and nonverbal clues sensations, mental images daydreamingcreativity

Some in-depth information about the Right Brain's Features and Functions

It enables young kids to comprehend the idea of more versus lessSome cognitive processes, including attention, processing of visual forms and patterns, emotions, language ambiguity, and implicit meanings, are controlled by the brain's right hemisphereRight brain functions predominately in children under the age of three

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ellow peas are dominant over green peas. Round peas are dominant wrinkled peas. Predict the outcome between two plants that are homozygous for BOTH traits.

Answers

You cannot be sure if the plant is homozygous or heterozygous as the data set is too small: by random chance, all three plants might have acquired only the dominant gene even if the recessive one is present.

Gene

A gene is the fundamental structural and functional component of heredity. The DNA that makes up genes. In some cases, genes serve as blueprints for the creation of proteins. In contrast, a large number of genes do not encode proteins. A few hundred DNA bases to more than 2 million bases make up the majority of human genes. Humans are thought to have between 20,000 and 25,000 genes, according to the Human Genome Project, a multinational effort to decipher the human genome's sequence and catalog its genes. Every gene is present in two copies, one from each parent, in every human being. Only a small subset of genes differ somewhat between individuals; the majority of genes are the same in all persons.

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Atherosclerosis, a particular type of arteriosclerosis, usually involves either complete blockage or severe restriction of blood flow due to high cholesterol and plaque buildup in the arteries. In extreme cases, constricted arteries may collapse from pressure applied outside of the arteries. This can best be explained by:

Answers

Atherosclerosis may worsen in the case where there is an increased velocity of the fluid in the already constricted sections of the arteries. This may lead to an increase in blood pressure.

Atherosclerosis is the condition where it leads to the arteries being thickened or hardened. The plaque is deposited on the walls of the artery. With the deposition in the arteries, the artery walls become thickened and lead to the reduction of the blood flow and supply of oxygen to body organs. When the vessels constrict, the flow of blood is decreased. The risk factors are increased blood pressure, obesity, and diabetes. The treatment options could be the consumption of anticoagulants, and medicines to lower blood pressure.

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Place the following in order of increasing entropy at 298 K.Ne Xe He Ar KrA) He < Kr < Ne < Ar < Xe.B) Xe < Kr < Ar < Ne < He.C) Ar < He < Ar < Ne < Kr.D) Ar < Ne < Xe < Kr < He.E) He < Ne < Ar < Kr < Xe. what type of contract is one in which both parties promise to perform their respective parts of an agreement in exchange for performance by the other party? Ethers with larger alkyl groups have higher boiling points due to O dipole-dipole interactions O ion-dipole interactions O ion-ion interactions O London dispersion forcesO hydrogen bonding bartk immigrated to ________ during the second world war and remained there for the last few years of his life a set of x and y scores has ssx = 21, ssy = 9, and sp = 55. what is the slope for the regression equation? round your answer to 2 decimal places. which entity gets a construction project started? a. construction firm b. bank c. owner d. regulatory agencies Direction: Draw a box() if it is an expression and a triangle (A) if it is an equation. 1. 2x + 9 =2. 32 + 3 x 9) = 593. 3k + 7 = 344. 5 (b + 28) = 1505. 9a + 7 = a metamorphic rock is formed around the magma chamber beneath a volcano. what range of pressures might you expect it to experience? what is the main purpose of the risk management plan? how should the document be constructed? How many moles are in 45.7 grams of calcium nitrite, Ca(NO2)2? Select one: O a. 2.03 moles b. 1.50 moles O c. 0.667 moles O d. 2.30 moles O e. 0.435 moles f. 0.486 moles g. 0.279 the cost of interest capital is an eva analysis is calcuated as the after-tax interest rate times ____. risc has a set of attributes that allows it to have a lower cycle per instruction (cpi) than a complex instruction set computer (cisc). true false which of the following roles do osteoblasts play in the remodeling process FILL IN THE BLANK. The goal of the organization's socialization efforts should be to minimize _______ as much as possible.a. mentoring b. diversity c. reality shock d. espoused values e. newcomer orientation I need help with the answer to this question Distinct layers that form in soil and can be distinguished from one another by appearance and chemical composition are referred to as ______ a two-mean nonpooled hypothesis test has two samples of sizes n1=17 and n2=24. the samples have standard deviations of s1=3 and s2=7. the degrees of freedom is found from the following calculation. mollusks are the second major phylum of the lophotrochozoa and include: after talking to her instructor, ada has questions about citing resources for her paper. what is her best course of action? time(s) if originally invoked with an argument of 0? Given the following code, factorial invoke itself___________ int factorial(int n){ if (n == 0) return 1: else return (n * factorial(n-1)); 1 3 0 2 5 2