Vmax = 499 μmol/min, Km = 2.34 mM, Total enzyme = 99.8 μmol.
What is the Vmax, Km, and total amount of enzyme present given substrate concentration and initial velocity data with a turnover number of 5000 min-1?To determine Vmax, we need to find the maximum initial velocity of the enzyme at saturating substrate concentration. From the given data, we can observe that the initial velocity reaches a plateau at substrate concentrations higher than 1000 mM.
Therefore, we can assume that the maximum initial velocity of the enzyme occurs at 4981 mM substrate concentration. Therefore,
Vmax = 499 μmol/min
To determine Km, we can use the Michaelis-Menten equation, which relates the initial velocity of an enzyme to the substrate concentration and the enzyme's kinetic constants.
V0 = Vmax [S] / (Km + [S])
We can rearrange this equation to obtain a linear equation that can be used to determine Km.
1/V0 = (Km/Vmax) * (1/[S]) + 1/Vmax
We can plot 1/V0 against 1/[S] and determine the slope and y-intercept of the resulting line. The slope will be Km/Vmax, and the y-intercept will be 1/Vmax.
Using the given data, we can calculate the values of 1/V0 and 1/[S].
[S] (mM) V0 (μmol/min) 1/V0 1/[S]
1 167 0.0059 1
2 250 0.004 0.5
4 334 0.003 0.25
6 376 0.0027 0.167
10 498 0.002 0.1
100 498 0.002 0.01
1000 499 0.002 0.001
4981 499 0.002 0.0002
We can then plot 1/V0 against 1/[S] and obtain a linear regression line.
plot of 1/V0 vs. 1/[S]
The slope of the line is 0.0047, which is Km/Vmax. Therefore,
Km = slope * Vmax = 0.0047 * 499 = 2.34 mM
To determine the total amount of enzyme present in these experiments, we need to know the units of enzyme activity that were measured. Assuming that the enzyme activity was measured in μmol/min, we can use the definition of turnover number (kcat) to determine the total amount of enzyme present.
kcat = Vmax / [E]
where [E] is the concentration of enzyme in the reaction mixture.
From the given turnover number, kcat = 5000 min^-1. Therefore,
[E] = Vmax / kcat = 499 / 5000 = 0.0998 μM
To determine the total amount of enzyme present, we need to know the total volume of the reaction mixture. Let's assume that the total volume was 1 mL. Therefore,
Total enzyme = [E] * volume = 0.0998 μM * 1 mL * 1000 μmol/μM = 99.8 μmol
Therefore, the total amount of enzyme present in these experiments is 99.8 μmol.
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how do you understand geological hazards?
Answer:
Geologic Hazards
Seismic hazards related to earthquakes, including ground rupture/faulting, liquefaction, strong motion, and tsunami.Landslides of all kinds, including seismically-triggered landslides, debris flows, mud flows, and rock falls.Mineral hazards such as asbestos, radon, and mercury.Explanation:
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Which of the following is an advantage angiosperms and gymnosperms have over mosses?
1. they are seed producing so can populate more areas
2. they can photosynthesize
3. they can use oxygen
4. they do not need water
Answer:
they are seed producing so can populate more areas
Explanation:
Which stage of mitosis occurs the least often and why?
Explanation:
The Anaphase stage is the shortest phase of mitosis. During this phase, disjunction occurs, and migration of sister chromatids away from each other to the poles of the cell occurs, leading to the formation of daughter chromosomes. Molecular motors use ATP to shorten the spindle fibers attached to each sister chromatid
Explanation:
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what happened to the
beak depth of finches born in 1978 compared to those born in 1976? Explain why you
think this occurred.
Answer:
Why do you think the average beak depth of the birds increased?
Explanation:
Because the drought reduced the number of seeds and finches with bigger beaks were able to eat the larger and harder seeds so more of them survived.
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ANYONE KNOW THIS?? PLZ LMK ASAP!!
Answer:
assuming this is human it is 46
Explanation:
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Does Genetic Modified Organism ( GMO ) make an apple bigger?
Answer:
the data only can determine the based on the replica of the fruit.
Explanation:
when fruit is genetically modified it's genetically is copied and the process of growing is sped up. in some since the fruit can change in size due to the result of multiple gene copies.
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What would happen to the members of a food web if most of the trees died?
A. only the trees will be effected
B. only things that eat tree leaves will have less to eat
C. Nothing will happen
D. The whole food web will be disrupted
1 point
Which explanation best identifies the relationship between mitosis and
reproduction? *
Mitosis is a form of sexual reproduction because the daughter cells form from one
single parent cell.
Mitosis is a form of sexual reproduction because the daughter cells are genetically
different from the parent cell.
Mitosis is a form of asexual reproduction because the daughter cells form a new
nuclear membrane after reproduction.
Mitosis is a form of asexual reproduction because the daughter cells are genetically
identical to the parent cell.
How does an organism obtain C6H12O6 and O2 to
undergo respiration?
Answer:
oxygen is given off during photosynthesis as a waste product
Explanation:
this is something that only plants and some algae and bacteria can do. they take sunlight and combined C02 and H20. they create glucose (C6H1206) and oxygen gas (02).
What is the mass of a box accelerating at 3 m/s^2 applying 36 N of force? Hint: Mass = force / acceleration
Answer:
I have attached a picture of me explaining how to find the mass. Hope this helps, thank you !!
Which statement is true regarding mitosis?
A. 2 cells are made from one cell
B. daughter cells have 1/2 of the original DNA
C. occurs only in sex cells
D. 4 cells are made from one cell.
Answer:
A. 2 cells are made from one cell
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How are the organs attached to the other body systems?
the body has levels of organisms that built on each other cells make up tissues, tissues make up organs, and organs make up organs system the function of an organs system
depends on the the integrated activity of it organs for instance digestive system organs corporate to process food
Cells in both plants and animals use ______ to create energy, carbon dioxide, and water, when breaking down glucose in the presences of oxygen.
A. Photosynthesis
B. ATP
C. Cellular Respiration
D. Fermentation
Answer:
C. Cellular Respiration
Answer:
Cells in both plants and animals use ______ to create energy, carbon dioxide, and water, when breaking down glucose in the presences of oxygen.
A. Photosynthesis
1. What are the different properties of light that apply to the image formation of
optical devices such as mirrors and lenses?
Answer:
Optical instruments are based on optics. They use mirrors and lenses to reflect and refract light and form images. The light microscope and telescope use convex lenses and mirrors to make enlarged images of very tiny or distant objects. A camera uses a convex lens to make a reduced image of an object.
The property of light that is important to the image formation in mirrors is reflection. The property of light that is important in the formation of an image by lenses is refraction.
Light refers more specifically to the portion of the electromagnetic spectrum that is perceivable with the optical eyes. There are several properties of light such as reflection, refraction, diffraction, interference, etc.
The property of light that is important to the image formation in mirrors is reflection. The property of light that is important in the formation of an image by lenses is refraction.
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How many times more erosion occurs in a soil with 3% moisture than a soil with 7% moisture is a 15 m s-1 wind?
Answer:
Twice.
Explanation:
If a soil with 3% moisture has twice the rate of erosion as compared to the soil having 7% moisture if both experience same speed of wind i. e. 15 m/s. This is because moisture held the particles of soil tightly with each other and prevent erosion in the soil so if the moisture becomes half in the soil then the rate of erosion is twice or doubled at constant wind speed.
The pituitary gland is most similar to:
A A worker in a factory
B A mail carrier
C The boss of a big office
D A security guard or police officer
Answer:
C. The boss of a big office
Explanation:
It tells all the other hormones what to do
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describe in on way in which the body gets rid of lactic acid
PLEASE HELP ASAP Match each word with the phrase that best defines it.
informal
a statement that is objective or unchanging
fact
done in a way that is friendly or casual
formal
a statement that is subjective or based on
interpretation
topic
done in a way that is suitable, appropriate,
or based on guidelines
opinion
the main point of a given subject
Answer:
fact: a statement that is objective or unchanging
informal: done in a way that is friendly or casual
formal: done in a way that is suitable, appropriate,
or based on guidelines
opinion: a statement that is subjective or based on
interpretation
topic: the main point of a given subject
Match each word with the phrase that best defines it.
1. Fact: a statement that is objective or unchanging
2. Informal: done in a way that is friendly or casual
3. Formal: done in a way that is suitable, appropriate, or based on guidelines
4. Opinion: a statement that is subjective or based on interpretation
5. Topic: the main point of a given subject
What are Phrases?A phrase is a group of words or a singular word which functions as a grammatical unit. For example, the English expression "the very happy boy" is a noun phrase containing the adjective phrase "very happy".
It can be a single word or a complete sentence. Phrases are used to describe the people, things, or events.
There are following types of phrases. They are:
Noun phrase.Adjective phrase.Adverb phrase.Verb phrase.Prepositional phrase.Gerund phraseInfinitive phraseParticipial phraseThus, matching the following words with its phrases
1. Fact: a statement that is objective or unchanging
2. Informal: done in a way that is friendly or casual
3. Formal: done in a way that is suitable, appropriate, or based on guidelines
4. Opinion: a statement that is subjective or based on interpretation
5. Topic: the main point of a given subject
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How do living organisms maintain homeostasis within their bodies?
Answer:
The body regulates those levels in an example of homeostasis. When levels decrease, the parathyroid releases hormones. If calcium levels become too high, the thyroid helps out by fixing calcium in the bones and lowering blood calcium levels. The nervous system helps keep homeostasis in breathing patterns.
Explanation:
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Answer:
Look below.
Explanation:
They maintain homeostasis by regulating temp, glucose, toxins, blood pressure, and pH.
What are 2 characteristics of index fossils?
In rabbits, straight ears (E) are dominant to floppy ears (e). Two rabbits are crossed, and 50% of their offspring have floppy ears. Which cross represents the genotypes of the parents?
Give the one chain to the following DNA AATAGGTACCCATGTGCA To write the complementary dna chain. How many nucleotides does this molecule have in total? How many are the bonds that unite nucleotides together?
Answer:
AATAGGTACCCATGTGCA (original DNA sequence)
TTATCCATGGGTACACGT
Explanation:
Adenine (A) pairs with Thymine (T). Guanine (G) pairs with Cytosine (C). There are 18 nucleotides in this sequence, as seen by the number of base pairs there are. Hydrogen bonds hold together the matching base pairs.
Nicole lives in the Arctic which has a temperature as low as –40°C.
(a) What class of food should Nicole consume continuously to adapt herself to the cold temperature?
Oats, bananas, red meats, but STAY AWAY from drugs & alcohol!
What is the difference between flu viruses and the measles virus?
Answer:Influenza and measles are viruses that both cause respiratory symptoms. The typical measles rash does not appear for 2-4 days, so during influenza season, symptoms of early measles can be confused with influenza.
Explanation:
Explanation:
Vaccines for both measles and flu already exist, however the flu vaccine must be updated because of the virus's ability to change and adapt in order to invade cells in the human body. The goal of researchers was to find genes in each virus that cannot survive changes.
By comparing two similar viruses which attack the body in similar ways through the lungs, researchers confirmed that the measles virus is much less open to evolutionary change by using an experimental technique called insertional mutagenesis to change the genes of each virus.
2. What might happen to the population of
rabbits if there were no foxes? sc.7.L.17.3
A
the population would increase
8 the population would decrease
© the population would stay the same
there would be no effect
D
i have no idea about this
Answer:
I think it's 4 or 3
Explanation:
Sorry if I'm wrong
When people living on hills put in concrete barriers, plywood fencing, and drainage pipes, they are trying ______________________
Answer: slow erosion down
Explanation:
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The lineage of pea plants produced round seeds for four generations. The plants that fertilized these pea plants also produced round seeds. However, in the fifth generation, the plant produced to two plants with wrinkled seeds. Can that be possible? Explain your answer using your pedigree chart.
Answer:
Explanation:
This happened because two fully recessive plants where introduced into the lineage. these plants where paired with the dominate verison. this would make all of the offspring have the two traits. this being said it would make it possible for the offspring to be Wrinkled Seeds.
When two heter0zyg0us individuals are crossed -simple d0minant inheritance-, or a heter0zyg0us individual is crossed with a h0m0zyg0us recessive one, they can produce h0m0zyg0us recessive subjects among the progeny. Heter0zyg0us individuals among the fourth generation got crossed with another plant (h0m0zyg0us recessive or heter0zyg0us) and produced two h0m0zyg0us recessive plants expressing wrinkle seeds.
---------------------------
The first step is to understand the given information. So,
The first four generations produced round seedsPlants that fertilized these plants also produced round seeds (see generation II) Among the fifth generation there are two plants with wrinkled seedsLet us assume that a diallelic gene determines the shape of seeds.
D0minant allele R codes for round seedsRecessive allele r codes for wrinkle seedsNow, let us analyze the pedigree. According to the information provided here, we can tell that
black figures represent h0m0zyg0us d0minant individuals, grey figures represent heter0zyg0us individualsempty figures represent h0m0zyg0us recessive individualsRemember that Generations are represented with roman numbers.
We will name the plants with numbers from 1 to 26.
According to the information in the statement and the information in the pedigree, we can say that grey individuals from the fourth generation are heter0zyg0us expressing round seeds. They can produce wrinkle seeds, if they are fertilized by another heter0zyg0us plant or a h0m0zygous recessive one.
Option 1:
Two heter0zyg0us plants are crossed, and their offspring have wrinkled seeds.
Option 2:
One heter0zyg0us plant is crossed with a h0m0zyg0us recessive one, and their offspring have wrinkled seeds. Let us see the cross
Look at the attached files for a better understanding
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Which statements describe how traits are inherited? Check all that apply. An offspring always receives different alleles from each parent. An offspring always receives the same alleles from each parent. An offspring can receive different alleles or the same alleles from each par Traits are inherited when proteins are passed from parents to offspring. Traits are inherited when genes are passed from parents to offspring.
Answer:
Traits are inherited when genes are passed from parents to offspring.
Explanation:
that is the definition of family traits
Answer:
An offspring can receive different alleles or the same alleles from each parent
and
Traits are inherited when genes are passed from parents to offspring
Explanation: